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Devices for Life Science Research |
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ProductsCircuSoft PFRETImages Digitized Video FRET (DVFRET) Microscopy: BFP-GFP-Pit-1 protein dimerization. The acceptor images
were acquired of doubly labeled HeLa cells expressed with both BFP-GFP-Pit-1 proteins. A - Unprocessed FRET;
F - Processed PFRET image
 Confocal FRET (C-FRET) Microscopy: FRET between Bcl-Xl and BAD in a vacuolated axon
(yellow arrows) 6 hours following impact acceleration injury. Panel A shows Bcl-Xl labeled with
Alexa 488. Panel B shows uncorrected FRET image (E=44%). Panel C shows corrected PFRET image.
The adjacent axon (yellow stars) does not demonstrate energy transfer.
 Two-Photon Excitation FRET (2P-FRET): Localization of CFP- and YFP-X/EBPB1 protein expressed in mouse 3T3 cells. The double expressed cells (CFP- and YFP-X/EBPB1) were excited 820nm, and the donor (D) and acceptor (uFRET) images of proteins localized in the nucleus of a single living cell were acquired. PFRET is the corrected image
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