CircuSoft Instrumentation
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CircuSoft PFRET - Precision FRET Data Analysis Software

Version 2.1 Now Avialable

See the new features added in version 2.1 here.

New in Version 2.0

• Process image stacks.
• Save and print false color images.

Features

• Open and save images in standard file formats including 8 bit TIFF, BMP, JPG.
• Supports 24-bit RGB images for FRET data processing (8 bits resolution per color).
• Display and Print images.
• Define single or multiple ROIs (region of interest) in one image.
• Statistics analysis including intensity histogram, minimum/maximum pixel values, number of pixels for certain pixel values, and mean of ROI.
• Image processing: background subtraction, arithmetic operation, and image crop.
• FRET image acquisition: remove SBT and correct FEL to obtain FRET image.
• Efficiency (%) and Distance (r) analysis: calculate efficiency and distance based in chosen ROIs (or whole image).
• Split images collected from older microscopy systems that do not have split channel functionality.
• Apply look-up tables for false coloring.