CircuSoft PFRET is a user-friendly software package that allows you to correct your FRET experiments for spectral bleed through, calculate FRET efficiency, and determine the distance between donor and acceptor molecules.
| Back Bleedthrough Correction | New function called complete correction from menu SingleSection_FRET-correction to correct extra contamination in FRET image in addition to donor bleedthrough and acceptor bleedthrough correction. For the details of the algorithm used in the correction, check the book chapter. |
| Bleedthrough Calibration | New function called Calibration from menu SingleSection_FRET to show bleedthrough ratio for different intensity ranges. This function provides information about how the bleedthrough ratio is distributed in your sample, whether it varies with different intensity range or keeps a constant ratio for different intensity ranges. By checking different control samples, it also provides information on whether the bleedthrough ratio is stable or not in order to give you idea whether the algorithm is right for your sample data. |
| Zoom In/ Out | From menu Image_View, two new function Zoom In, Zoom Out are added. |
| Show all ROIs on an image | In the image detail window, a new command Show All ROIs to display all chosen ROIs on the image is available. |
| FRET image acquisition: remove SBT and correct FEL to obtain FRET image. |
| Efficiency (%) and Distance (r) analysis: calculate efficiency and distance based in chosen ROIs (or whole image). |
| Process image stacks. |
| Save and print false color images. |
| Open and save images in standard file formats including 8 bit TIFF, BMP, JPG. |
| Supports 24-bit RGB images for FRET data processing (8 bits resolution per color). |
| Display and Print images. |
| Define single or multiple ROIs (region of interest) in one image. |
| Statistics analysis including intensity histogram, minimum/maximum pixel values, number of pixels for certain pixel values, and mean of ROI. |
| Image processing: background subtraction, arithmetic operation, and image crop. |
| Split images collected from older microscopy systems that do not have split channel functionality. |
| Apply look-up tables for false coloring. |
| 05/31/05 | PFRET v2.2 Released | PFRET |
| 09/21/04 | PFRET v2.1 update available | PFRET |